Coding

Part:BBa_K4623009:Design

Designed by: Ruixiao Tan   Group: iGEM23_BNU-China   (2023-10-09)


Twisted Silinker,Silica-protein junctions that curving in response to calcium ions


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 955
    Illegal EcoRI site found at 1123
    Illegal EcoRI site found at 1345
    Illegal PstI site found at 1066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 955
    Illegal EcoRI site found at 1123
    Illegal EcoRI site found at 1345
    Illegal PstI site found at 1066
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 955
    Illegal EcoRI site found at 1123
    Illegal EcoRI site found at 1345
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 955
    Illegal EcoRI site found at 1123
    Illegal EcoRI site found at 1345
    Illegal PstI site found at 1066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 955
    Illegal EcoRI site found at 1123
    Illegal EcoRI site found at 1345
    Illegal PstI site found at 1066
    Illegal AgeI site found at 445
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 940


Design Notes

The sequence includes the stop codon.CaM and CBP sequences can be replaced by other protein sequences that bind to each other in response to signals.


Source

This part sequence is primarily derived from an artificially modified amino acid sequence that has been optimized based on E. coli codons.

References